Short Tandem Repeat Kit

Short tandem repeat polymorphisms strps are small tracts of tandemly repeated dna sequences that are polymorphic and generally of high heterozygosity in the human population. The repeats can range in length from 2 to 6 base pairs repeat. Strs short tandem repeat have become popular dna repeat markers because they are easily amplified by the polymerase chain reaction pcr without the problems of differential amplification.

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These repeated sequences come in various sizes and are classified according to the length of the core repeat units the number of contiguous repeat units and or the overall length of the repeat region.

Short tandem repeat kit. Usually this method is applied in cases of male on female sexual assault in which the victim s dna is in great excess and masked the male contribution. Short tandem repeat str typing methods are widely used today for human identity testing applications including forensic dna analysis. By designing pcr primers to locus specific telomeric strps the sequence across the repeats can be amplified and the allele sizes measured. Short tandem repeats strs the human genome is full of repeated dna sequences.

Short tandem repeats strs are repeated dna motifs consisting of 2 to 13 nucleotides. Croatian medical journal 50 268 285. A short tandem repeat str or microsatellite is a pattern of two or more nucleotides that are repeated directly adjacent to each other. To develop a reagent kit that enables multiplex polymerase chain reaction pcr amplification of 18 short tandem repeats str and the canine sex determining zinc finger marker validation studies to determine the robustness and reliability in forensic.

Denise s et al. Uses for str analysis include cell line authentication human biobank sample matching and ex vivo cell treatment tracking. A short tandem repeat polymorphism occurs when homologous str loci differ in the number of repeats between individuals. The analysis of short tandem repeat str markers located on the y chromosome is an established method in forensic casework analysis.

Str analysis measures the exact number of repeated units and compares specific loci on dna from two or more samples. Following multiplex pcr amplification dna samples containing the length variant str alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. Developmental validation of short tandem repeat reagent kit for forensic dna profiling of canine biological material. Power of exclusion for parentage verification and probability of match for identity in american kennel club breeds using 17 canine.

Dayton m et al.