Yessotoxin Tandem Mass Spectrometry

However the applicability of this approach for analyzing metabolites at physiologically.

Yessotoxin tandem mass spectrometry. Tandem mass spectrometry ms ms tandem ms or ms ms msn is a technique to break down selected ions precursor ions into fragments product ions. Ceuv is a good method for ytx analysis as its selectivity can easily differentiate between ytxs and dsp toxins. Pectenotoxins 2 ptx 2 11 ptx 11 2 seco acid ptx 2sa and yessotoxin ytx were identified by comparison of retention times and collision induced mass spectra of certified standards and field sample extracts. The fragments then reveal aspects of the chemical structure of the precursor ion.

Labeled yessotoxin obtained from these experiments was subjected to collision induced dissociation tandem mass spectrometry to determine the positions of 18o incorporation pattern in the molecule. The technique gives a limit of detection lod of 0 3 μg ml and a limit of quantification loq of 0 9 μg ml. It is much less toxic by gavage with no deaths being recorded at doses up to 54 mg kg 20. The techniques used for ytx analysis include.

Diagnostic and predictable gas phase ion molecule reactions have emerged as a potential alternative to collision activated dissociation in tandem mass spectrometry ms2 experiments performed to gain structural information for unknown organic compounds such as drug metabolites in complex mixtures. Detailed analyses of product ions from the fragmentation processes led to the identification of 18o labeled positions and the incorporation ratios. Tandem mass spectrometry also known as ms ms or ms 2 is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. Ce with ultraviolet uv detection and ce coupled to mass spectrometry ms.

The sensitivity of these techniques can however be poor due to the low molar absorptivity of the analytes. A common use of tandem ms is the analysis of biomolecules such as proteins and peptides. This is the first report of ptxs and ytx from planktonic samples in chilean coastal waters. To enable the development of a tandem mass spectrometry ms ms based methodology for selective protein identification and differential quantitative analysis a novel derivatization strategy is proposed based on the formation of a fixed charge sulfonium ion on the side chain of a methionine amino acid residue contained within a protein or peptide of interest.

Liquid chromatography mass spectrometry lc ms ms further investigations on those samples that gave positive results by the mba for lipophilic toxins were carried out by liquid chromatography tandem mass spectrometry.